Imagej fiji download

Author: i | 2025-04-24

Fiji; Fiji ( Fiji Is Just ImageJ ) Fiji is an open source image processing package based on ImageJ. Fiji's main purpose is to provide a distribution of ImageJ with many bundled plugins.

Fiji ( Fiji Is Just ImageJ )

除了 ImageJ，大家应该还听说过 Fiij（Fiij is just Image J ）、Image pro plus。如果你不知道这三者该选哪一个作为你的生物图像分析处理软件，不妨看看以下对这三个软件的对比分析。文章内也分享了安装教程，三个工具的安装包以及工具的配套教程打包好了，有需要在福麦斯生物公众hao回复“实验06”就能下载。一、Image J 1、ImageJ 介绍基于强大的图片分析与处理功能，Image J在科研中的应用极为广泛。2021年被Nature评为十大影响科学的代码。最关键的，这款软件是完全免费！ImageJ 是一个基于 java 的公共的图像处理软件，能够显示，编辑，分析，处理，保存，打印 8 位，16 位，32 位的图片， 支持 TIFF，PNG， GIF， JPEG， BMP， DICOM， FITS 等多种格式。支持图像栈（stack）功能，即在一个窗口里以多线程的形式层叠多个图像， 并行处理。只要内存允许，ImageJ 能打开任意多的图像进行处理。除了基本的图像操作， 比如缩放，旋转， 扭曲， 平滑处理外，ImageJ 还能进行图片的区域和像素统计，间距，角度计算，能创建柱状图和剖面图，进行傅里叶变换。ImageJ 的科研应用：①免疫组织化学/原位杂交目的区域光密度分析② 共聚焦图像中两种蛋白共定位的程度（相关系数计算）③免疫荧光图像分析:计数兴趣区大小、阳性点密度、数量，结构长度分析④ 高尔基染色的神经元或者共聚焦显微图像中表达EGFP等神经元突起（树突、轴突）长度测量，Sholl分析，突触棘计数⑤阳性细胞技术等等...ImageJ 的优点：免费（不用担心知识产权问题），程序小，运行快，操作简单，不断有新的插件加入，非常适用于经费有限的实验室以及初入门的神经科学研究生。如果只需要一些基础的操作，例如Western Blot条带定量、荧光定量等，推荐大家选择ImageJ。如果基本功能不满足，也可以自行安装其它插件。小tips：如果需要使用的插件比较多（正好你还是生命科学领域的研究人员），更推荐下面说到的fiji（fijiis just ImageJ），它是ImageJ的版本之一。详细介绍可看下文对fiji的介绍。2、lmageJ 安装lmageJ 可运行于 Microsoft Windows，Mac OS，Mac OS X，Linux，和 Sharp Zaurus PDA 等多种平台。安装包文章开头分享了。根据电脑系统选择的安装包，双击安装文件之后开始安装，安装步骤非常简单，这里就不做分享了。注意：要在预装JAVA的计算机上安装lmageJ。二、fiji is just ImageJ1、fiji介绍fiji 是 ImageJ 的版本之一，全称为 fiji is just ImageJ。fiji 就是预装了很多生物医学分析常用插件的ImageJ。如果不想手动安装插件，更推荐你安装 fiji。fiji 由于预装的插件非常丰富（如神经元追踪、荧光共定位等），所以安装包略大一些，打开速度也会略慢。fiji 和 ImageJ 的区别：fiji 里绑定的命令和插件超过五百个，目标用户是生命科学领域的研究人员，为此捆绑了很多该领域用户会经常用到的插件。而 ImageJ 需要手动安装插件。另外对于 fiji 里绑定的超过五百个命令和插件，你不需要记住他们在哪个菜单和子菜单里，只要在搜索栏里输入命令的名字，就可以直接调用。做个小补充：fiji 不是一个全新的软件，只是 ImageJ 的一个针对生命科学用户的发布版本。但是 fiji 不是 ImageJ 唯一的发布版本。比如，面向天文学研究人员的SalsaJ，用于显微镜控制和图像获取的 µManager 等。用一句话概括的话，fiji is just imageJ， but ImageJ is not just fiji.2、fiji 安装安装包在文章开头分享了。fiji 在 macosx、windows（7以及7以上的版本）、Linux (64-bit) 上都能使用。根据自己的电脑系统选择对应的安装包进行解压。如windows 64位就选择fiji-win64.zip进行解压，解压之后点击ImageJ-win64exe，fiji直接双击就能打开软件使用，没有安装步骤。注意：下载好安装包之后不要把安装包保存在C盘，同时保存到磁盘的文件夹命名不要出现中文字，否则软件会出现出发打开或者闪退的现象。3、fiji 闪退的解决办法①可能你的fiji is just ImageJ的储存目录有中文命名的文件夹。就是你解压路径中不包含中文名称目录，并且不要解压在“program files”路径中。②不要把安装包放在C盘。③fiji is just ImageJ需要Java环境，如果闪退那么可能是你的电脑Java版本太低。Java下载地址如下：Java SE Development Kit： ImageJ 和 fiji 的介绍和对比之后，相信你对于这两个版本该如何选择，心里应该有了答案！如果你是生命科学领域的从业者，选 fiji 就好了。没有特殊的需求，它绑定的插件足够你使用，之后就不需要你自己再手动安装插件。什么情况下需要选择 ImageJ（ImageJ1.X）？① ImageJ 菜单里的基本功能就能满足你的要求，不需要其他插件，这种情况下可以选择单独的ImageJ（ImageJ1.X）。因为体积比fiji小，它的启动速度也比 fiji 要快，这也是使用ImageJ的之一。②有一些早期开发的 ImageJ 插件，只提供了后缀为.java的文件。这种情况下可以用ImageJ的plugins > compile and run命令将之编译为.class文件，然后再放到 fiji 里使用。但这种方法也有失灵的时候，这种情况下就只能在ImageJ里使用该插件了。四、Image pro plus1、Image pro plus 介绍除了ImageJ和fiji，还有一个 Image pro plus，身边也有很多大佬都在用这个软件。或许你也听说过这个软件。Image-Pro Plus 也是一款用于科学图像处理和分析的软件，它是 Image-Pro 软件系列的一部分，广泛应用于医学、生物学、材料科学和工程学等领域，可与其他软件和设备进行无缝集成。由于 Image-Pro Plus 是商业软件，用户需要购买许可证才能使用。文末分享了 image pro plus 的破解版安装包免费使用（仅供学习使用）。image pro plus 的功能包括：图像增强和校正功能图像分割和测量工具图像拼接，图像配准和图像融合，可用于创建全景图像和多模态图像跟踪和测量运动对象，生成动态图像直方图分析，图像分类，颜色分析和形状分析等自动化处理流程和批量处理大量图像数据Image pro plus 的科研应用：合成荧光图片、免疫组化图片光密度测量、凝胶电泳图片的测量、凝胶电泳图片的测量、荧光强度测量、测量鼠脑切片的梗塞面积测量心肌纤维截面积、找两张图片的差异之处、测量划痕实验的细胞间距、测量网状的细胞壁等等。2、Image pro plus 安装安装包分享在文章开头了解压文件，确保软件所放置的路径里没有中文（既所有文件夹名字里都没有中文，分区名字也没有中文，如有同学喜欢把分区命名为“软件”什么的，改成英文即可），暂时关闭 360 等卫士。以前如果装过Image-Pro Plus 需要先卸载。打开【ipp6.0】文件夹，双击运行【start】文件点击 Install Image-Pro Plus 开始安装按照默认路径安装(不能修改，一定要按默认安装在c 盘，否则会导致软件不可使用)。选Typical 安装，遇到要填名称和单位的界面，随便填即可。一路 next 即可。有部分笔记本电脑(如华硕)会出现 EnTech Taiwan 驱动的错误提示，这是电脑触摸板的驱动，点关闭就行。打开【crack 文件】文件夹，复制里面三个文件，覆盖软件安装路径下（C:\IPWIN60）的文件。运行【ipwin32】即可打开软件，选 complete。弹出的注册对话框，勾选不再提醒，点 remind me later 即可。不用注册(其实已注册)。另一个对话框也是关掉就行。五、imagej和image pro plus如何选择？Image-Pro Plus 与 ImageJ均具有强大的图像分析功能。总体来看，更推荐Image J。①在标定方面，Image-Pro Plus 标定方法及具体操作与 Image J 无明显差别；②在 ROI 选择及基本参数测试方面，Image-Pro Plus 的操作选择方式及备选参数数量较Image J丰富；③在添加插件功能方面，Image J 可供下载及利用的插件数量远远多于 Image-Pro Plus；④在操作实现方面，ImagePro Plus 的一些常用操作，如 ROI 选择，参数测试等较 Image J 复杂。⑤image pro plus 其实和 ImageJ 分析的指标都差不多，但是 image pro plus 操作起会更复杂，对初学者来说不太实用。⑥从费用上来说，实验室资金充足完全可以购买 image pro plus 这类收费软件。但是相比起来 ImageJ 的免费使用就更经济实惠。如何选择？①当 Image J 和 Image-Pro Plus 基本功能都能满足测试要求时，首选 Image J。②如果图像分割困难，或不想借助插件而能测试较多的参数，推荐 Image-Pro Plus 为首选图像分析软件。③如果 Image-Pro Plus 和 Image J 软件的基本功能不足以满足具体测试需求，需通过添加插件来扩展功能，或者是直接安装 fiji。以上就是对于 Image J、fiji、Image pro plus 三个生物图像分析处理软件的详细介绍，希望能对你有所帮助！科研资源汇总扫描下方【福麦斯生物】公众hao二维码，回复对应的资源关键词就能在公众号下载资源。举个栗子：如果你想下载endnote安装包，你就在【福麦斯生物】公众hao回复：软件314，之后会自动弹出安装包。endnote、zotero、spss、GraphPadPrism都有激活版的安装包！顶级图像分析工具，ImageJ、Fiji、Image pro plus，选这3款准没错！科研绘图王炸：11款矢量素材免费网站/软件合集！画科研插图一定要选ScienceSlides！好用到离谱！打包科研大佬的绘图软件，使用频率最高的Top 9安装！（带安装包）OriginPro 2022，更多拓展图形模板！GraphPad Prism 9.3 英文版，（含激活码）+软件教程 白嫖这12个插件，让你的Zotero成为地表最强文献管理器！ EndNote X9突然提示要激活？最新解决办法来了！Adobe Acrobat Pro 2023，科研人必备的PDF编辑器！ StyleWriter最新白嫖方案 | 神器在手！润色不愁！附报错解决 润色出神仙SCI，WhiteSmoke来了 引物设计神器Oligo 6、Oligo 7安装教程（带安装包）引物设计神器！Primer Premier 5&6安装分项（带安装包）Primer Premier 6，3分钟教你完成引物设计（含软件安装）SnapGene 6.02，DNA可视化，分子克隆、序列编辑就用它！（可切换中文）

Fiji ( Fiji Is Just ImageJ ) - Brown University

Why can't I install Fiji Maps And Direction?The installation of Fiji Maps And Direction may fail because of the lack of device storage, poor network connection, or the compatibility of your Android device. Therefore, please check the minimum requirements first to make sure Fiji Maps And Direction is compatible with your phone.How to download Fiji Maps And Direction old versions?APKPure provides the latest version and all the older versions of Fiji Maps And Direction. You can download any version you want from here: All Versions of Fiji Maps And DirectionWhat's the file size of Fiji Maps And Direction?Fiji Maps And Direction takes up around 4.2 MB of storage. It's recommended to download APKPure App to install Fiji Maps And Direction successfully on your mobile device with faster speed.What language does Fiji Maps And Direction support?Fiji Maps And Direction supports isiZulu,中文,Việt Nam, and more languages. Go to More Info to know all the languages Fiji Maps And Direction supports.

fiji/fiji: A batteries-included distribution of ImageJ

HXTT Word 1.0.030 Pure Java Type 4 Word JDBC(4.2, 4.3) driver packages for ... correlated subquery. The drivers are completely written in Java and can be deployed on any platform with Java VM, which includes Microsoft Windows, Novell Netware, Apple ... Shareware | $339.00 IETester 0.5.4 ... that allows you to have the rendering and javascript engines of IE11, IE10, IE9, IE8, IE7 IE 6 and IE5.5 on Windows 8 desktop, Windows 7, Vista and XP, as ... Freeware IIPImageServer 0.9.9 ... Nginx etc) Several clients available – Ajax, flash, java applet etc Instant dynamic generation of JPEG overviews or details at any resolution Allows easy viewing of extremely large images (gigapixel ... Open Source ImageJ 1.54i ImageJ is an interesting Java based image processing application inspired by NIH Image ... a downloadable application, on any computer with a Java 1.1 or later virtual machine. It can display, ... with an open architecture that provides extensibility via Java plugins. Custom acquisition, analysis and processing plugins can ... Freeware ImageJ x64 1.54i ImageJ x64 is an intersting Java based image processing application inspired by NIH Image ... a downloadable application, on any computer with a Java 1.1 or later virtual machine. It can display, ... Freeware ImageMagick for Windows (x64 bit) 7.1.1-34 Introduction to ImageMagick ImageMagick for Windows x64 is a software suite to create, edit, and compose bitmap images. It can read, convert and write images in a variety of formats ... Freeware INDI for Java 1.31 Beta INDI for Java was specially created as an Open Source Java library that manages to implement the INDI distributed control protocol. INDI for Java has been designed to easily implement new INDI ... Open Source InnoList 1.3 Use InnoList for custom-fit management and organisation of your data. With the help of a easy graphical user interface you create tables and forms that match the actual needs. No database ... Shareware | $45.00 install4j 11.0.0 ... seeking to deliver seamless installation experiences for their Java applications. This powerful tool stands out for its ... empowers developers to create professional-grade installers for their Java applications. Its combination of intuitive design, extensive customization ... Trialware install4j Portable 11.0.0 ... install4j Portable is a powerful and versatile multi-platform Java installer builder developed by ej-technologies GmbH. This software ... macOS, Linux, and Unix. This ensures that your Java applications can be deployed seamlessly across different environments, ... Trialware install4j x64 10.0.6 ... own personalized native installers and application launchers for Java applications. install4j excels in its ease of ... X Installers and Uninstallers: · Support for Java 1.3, 1.4, 1.5 and 1.6 · LZMA and ... Trialware | $699.00 InstallAware Express MSI Installer X6 ... Then make refinements using InstallAware's intuitive task-based views. Java Installation Support Build Java application installations, with support for preinstalling Java Virtual Machines and runtime environments on Windows systems. ... Shareware | $499.00 tags: msi builder, msi tool, msi tools, msi, build msi, msi package builder, appx builder, app-v builder, web install, desktop. Fiji; Fiji ( Fiji Is Just ImageJ ) Fiji is an open source image processing package based on ImageJ. Fiji's main purpose is to provide a distribution of ImageJ with many bundled plugins. Fiji; Fiji ( Fiji Is Just ImageJ ) Fiji is an open source image processing package based on ImageJ. Fiji's main purpose is to provide a distribution of ImageJ with many bundled plugins.

Fiji: ImageJ, with Batteries Included

Images of western blot are presented in supplementary figure (S1). Immunohistochemical staining for OPN showed significant increase in WT and meprin βKO kidney tubules (D) and renal corpuscles of WT and meprin βKO (E). OD data were quantified (n = 3) using Image J analysis Software (ImageJ/Fiji 1.46) and analyzed for 10 non-overlapping fields from tubular and renal corpuscle sections from each kidney. Images at 60× magnification and the scale bar representing 20 μm. Immunofluorescence counterstaining of OPN (red) with either meprin β (green) in WT mice or villin (PT marker, green) in meprin β-deficient mice showed that meprin β induced expression of OPN in PTs in kidneys subjected to IR (F). DAPI was used to stain the nuclei (blue). Images at 60× magnification and the scale bar representing 20 μm. Data is expressed as mean ± SEM with P values as indicated, P ≤ 0.05 are considered statistically significantFull size imageMeprin β expression was associated with increased expressions and shedding of osteopontin into proximal kidney tubulesA previous in vitro study demonstrated that meprin β is capable of proteolytically processing OPN [7]. To determine the impact of meprin β expression on OPN levels in vivo, mRNA and protein expression of OPN were evaluated. Real-time PCR data showed that the mRNA levels for OPN increased significantly in both WT (P ≤ 0.01) and βKO (P ≤ 0.05) mice at 24 h post-IR (Fig. 1B). Western blot analysis detected a full length OPN protein band at 66 kDa, with significant increases (P ≤ 0.05) in protein levels in WT at 24 h post-IR when compared to levels in control kidneys. This increase in OPN protein levels was not observed in the kidneys of βKO mice subjected to IR (Fig. 1C). Additionally, we detected a cleaved OPN fragment at 32 kDa, for which there were no significant changes in either genotype at 24 h IR post-IR (Fig. 1C). We used immunohistochemical staining and light microscopy to assess the localization of OPN in kidney sections and quantified the staining intensity using Image J. Our data showed a significant increase in the staining intensity of OPN in select tubules from WT (P ≤ 0.0001) and a modest increase in βKO (P ≤ 0.05) tubules at 24 h post-IR (Fig. 1D). Interestingly, the staining intensity for OPN in renal corpuscles significantly increased in both WT (P ≤ 0.001) and βKO (P ≤ 0.001) at 24 h

Fiji ( Fiji Is Just ImageJ )

The real-time PCR data showed that mRNA expression levels of Caspase-3 decreased in WT and increased in βKO mice at 24 h post-IR (A). Caspase-3 mRNA levels are expressed as fold change (FC) relative to the WT control and normalized to GAPDH mRNA and each value represents the mean ± SEM of triplicate combinations from 4 mice per group. Western blot analysis detected two bands of Caspase-3 (33 and 35 kDa) with significant increase in the Caspase-3 (33 kDa) band for WT kidneys only (B). The protein bands represent samples from individual kidneys (n = 3). The relative optic densities (ODs) were calculated by normalizing the ODs of Caspase-3 to the ODs for β-tubulin in the same sample. The full-length images of western blot are presented in supplementary figure (S2). Immunohistochemical staining of Caspase-3 showed significant increase in select tubules from WT kidney only (C) and renal corpuscles of both genotypes (D). OD data were quantified (n = 3) using Image J analysis Software (ImageJ/Fiji 1.46) and analyzed for 10 non-overlapping fields from tubular and renal corpuscle sections. Images at 60× magnification and the scale bar representing 20 μm. Immunofluorescence counterstaining of Caspase-3 (red) with either meprin β (green) in WT or villin (PT marker, green) in meprin β-deficient mice showed that Caspase-3 increased in both PTs and DTs of WT but not in meprin βKO mice (E). DAPI was used to stain the nuclei (blue). Images at 60× magnification and the scale bar representing 20 μm. Data is expressed as mean ± SEM with P values as indicated, P ≤ 0.05 are considered statistically significantFull size imageWe used a similar approach to evaluate the expression of the anti-apoptotic and cell survival gene Bcl-2. Real-time PCR analysis showed no significant change in Bcl-2 mRNA levels in WT but significant increases in βKO kidneys (P ≤ 0.05) at 24 h post-IR, (Fig. 3A). Interestingly, western blot analysis showed opposite patterns with a significant increase (P ≤ 0.05) in Bcl-2 protein levels in WT kidneys at 24 h post-IR, but not in βKO mice (Fig. 3B). Immunohistochemical staining coupled with light microscopy analysis showed that the increase in protein level for Bcl-2 in WT kidney tubules was not global, but only in select tubules (P ≤ 0.0001). For βKO kidneys, the levels were significantly higher (P ≤ 0.01) in select kidney tubules as well as interstitial cells (presumed to be macrophages/dendritic cells)

Fiji ( Fiji Is Just ImageJ ) - Brown University

Please use version inside folder "03-18-2016_release".Instructions:1. Place all files inside the "jars" folder inside the following directory: Program Files > ImageJ > plugins2. Simply drag moco_.jar onto ImageJ.Alternatively (if that doesn't work for some reason),Open ImageJ, clickPlugins > Install Plugin...and choose 10092015_release>moco_.jar.3. Restart ImageJ, and and the moco plugin should show up under Plugins.---------------------------------Directions: You must have open the stack and the template image that you want to align the stack with (i.e. the first image in the stack, or the average image of all frames in the stack).w: The maximimum distance (in pixels) to be translated in the x and y directions. (i.e. w = 2 means that the maximum translation can be 2 pixels up/downand 2 pixels left/right).Downsample value: The amount of times to downsample by 2 for faster processing. (i.e. downsampling 512x512 image by 1 makes it 256x256). Leads to problems if downsample value is too large.Generate log file: Generates a results table that shows the amount by which each image was translated. Can be saved and used for other images/channels.Plot: Plots the sqrt(x^2 + y^2), i.e. the amount of total translation for each frame(to plot RMS, you must also choose to generate the log file).If an image in the stack to be registered is translated by amounts that seem too large, then the program will warn you and will allow you to normalize the intensity in the image (which can improve results).We recommend that you use 8-bit stacks for registration (there are sometimes issues with 16-bit stacks that lead to translation terms that are too large as noted above). If you generate a log file and save it, you can use it later to register the original 16-bit stack really fast. Email guevara.james@gmail.com to ask any questions or report any bugs or feature requests.

fiji/fiji: A batteries-included distribution of ImageJ

(Fig. 3C). Staining intensity for Bcl-2 in renal corpuscle tissue increased significantly in both WT (P ≤ 0.05) and βKO (P ≤ 0.0001) (Fig. 3D). To assess the localization of synthesized Bcl-2 in kidney tubules, we used immunofluorescence, counterstaining with villin a proximal tubule marker in both genotypes [27]. Our data showed that Bcl-2 expression levels increased in both proximal and distal renal tubules for WT kidneys. In βKO kidneys, Bcl-2 levels increased in both renal tubules and interstitial tissues (Fig. 3C). The increase in Bcl-2 expression in both WT and βKO kidney tissues suggests a partial association to meprin β expression. This leads us to conclude that meprin β has a modest impact on Bcl-2 anti-apoptotic effect; therefore, other mediators could be involved.Fig. 3Bcl-2 expression in kidney tissue from WT and meprin βKO mice at 0 h and 24 h post-IR. The real-time PCR data showed that mRNA expression levels of Bcl-2 significantly increased in βKO mice only at 24 h post-IR (A). Real-time-PCR analysis showed significant increases in Bcl-2 mRNA levels in both genotypes at 24 h post-IR. Bcl-2 mRNA levels are expressed as fold change (FC) relative to the WT control and normalized to GAPDH mRNA and each value represents the mean ± SEM of triplicate combinations from 4 mice per group. Western blot analysis showed a significant increase of the Bcl-2 protein level in WT at 24 h post-IR but not in βKO mice (B). The protein bands of Bcl-2 detected at 26 KDa represent samples from individual kidneys (n = 3). The relative optic densities (ODs) were calculated by normalizing the ODs of Bcl-2 to the ODs for β-tubulin in the same sample. The full-length images of western blot are presented in supplementary figure (S3). Immunostaining of Bcl-2 showed significant increase in select tubules of both genotypes (C) and renal corpuscles (D). OD data were quantified (n = 3) using Image J analysis Software (ImageJ/Fiji 1.46) and analyzed for 10 non-overlapping fields from tubular and renal corpuscle sections. Images at 60× magnification and the scale bar representing 20 μm. Representative immunofluorescence staining for Bcl-2 (red) in WT and meprin βKO mice kidney tubules showed that levels of Bcl-2 increased in both proximal and distal tubules for WT kidneys (E). Villin (green) was used as a proximal tubule marker in both genotypes because of antibody incompatibility. DAPI was used to stain the nuclei (blue). Images at. Fiji; Fiji ( Fiji Is Just ImageJ ) Fiji is an open source image processing package based on ImageJ. Fiji's main purpose is to provide a distribution of ImageJ with many bundled plugins. Fiji; Fiji ( Fiji Is Just ImageJ ) Fiji is an open source image processing package based on ImageJ. Fiji's main purpose is to provide a distribution of ImageJ with many bundled plugins.

Fiji: ImageJ, with Batteries Included

And staining intensity for NFκB increased significantly only in WT kidneys renal corpuscles of both genotypes (D). OD data were quantified (n = 3) using Image J analysis Software (ImageJ/Fiji 1.46) and analyzed for 10 non-overlapping fields from tubular and renal corpuscle sections. Images at 60× magnification and the scale bar representing 20 μm. Immunofluorescence counterstaining of NFκB (red) with either meprin β (green) in WT or villin (PT marker, green) in in meprin β-deficient mice showed increases in levels of NFκB in proximal tubules of WT kidneys (E). DAPI was used to stain the nuclei (blue). Images at 60× magnification and the scale bar representing 20 μm. Data is expressed as mean ± SEM with P values as indicated, P ≤ 0.05 are considered statistically significantFull size imageDiscussionExpression levels and localizations of meprin metalloproteases has been shown to be associated with IR-induced kidney injury [2,3,4, 27]. Meprins are redistributed from the BBM to the cytoplasm and basolateral compartments of proximal tubule epithelial cells in IR-induced kidney injury [2], allowing the enzymes to interact with proteins present in these cell compartments. Meprin B has been shown to proteolytically process osteopontin (OPN) [7, 31] a promising candidate that has demonstrated significance as a potential biomarker in several forms of kidney disease [8, 9, 32,33,34,35,36,37,38]. OPN is a highly phosphorylated multifunctional protein that plays an important role in kidney diseases such as IR-induced acute renal injury, interstitial inflammation, and fibrosis [9]. Several studies demonstrated that inhibiting OPN expression or activity can alleviate kidney injury and enhance overall kidney function through its multifunctional roles (e.g. enhances macrophage and T cells infiltration and cytokine synthesis) [39, 40]. Although OPN is recognized for promoting inflammation and cell proliferation, it has also been shown to attenuate apoptosis [41, 42], a process regulated by key apoptotic proteins, namely Bcl-2, Bax, and Caspase-3. In addition, OPN promotes activation of NF-kappaB (NFκB), a key protein that plays a vital role in regulating apoptosis [43, 44] and promotes apoptosis especially in response to cellular stress [45,46,47], enhancing pro-inflammatory responses that can lead to tissue damage, particularly in ischemia/reperfusion (IR) injury [40]. By promoting the expression of NFκB target genes, OPN can amplify inflammation and contribute to tissue damage [12, 14, 48], consequently regulating the apoptotic pathway. Upon NFκB activation in response to cellular stress, such as oxidative stress or tissue injury commonly seen in IR conditions [45,46,47], OPN exhibits an

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1:400, and rabbit monoclonal anti- NFκB (Cell Signaling Technology Cat# 8242, RRID: AB_10859369) diluted 1:400.For standard immunostaining, we used the Vectastain® Elite® ABC Universal Kit Protocol (Vector Laboratories, Burlingame CA) following the manufacturer’s instructions, with peroxidase substrate solution (DAB) (Vector Laboratories Cat# SK-4100, RRID: AB_2336382) applied to obtain brown staining. The sections were counterstained with hematoxylin (to stain the nuclei), washed and mounted with coverslips and left at room temperature overnight to dry.The staining intensity of the tissue sections for the various proteins (OPN, Caspase-3, Bcl-2, and NFκB) were evaluated using BZ-X700 Series all-in-one fluorescence (KEYENCE Corporation of America, Elmwood, NJ) and imaged using BZ-X700 analysis Software. Optical density (OD) was determined in ten (10) non-overlapping fields for tubular and ten non-overlapping fields for renal corpuscles at 60 X magnification from each section. The fields from the sections were obtained in a blinded manner and quantified using 8-bit images for calibrated OD values and OD standard using Image J analysis Software (ImageJ/Fiji 1.46).Immunofluorescence stainingTo determine localization of the proteins of interest, we used immunofluorescence counterstaining according to the previously described protocols [6, 21] with two proximal tubule markers, meprin β in WT kidney sections (Goat Anti Mouse Meprin β Subunit/MEP1B Antigen Affinity purified Polyclonal Antibody (R and D Systems, Cat # AF3300, RRID: AB_2143451) diluted 1:100 and villin in βKO kidney sections (mouse monoclonal anti-villin antibodies, Santa Cruz Biotechnology Cat# sc-58897, RRID: AB_2304475) diluted 1:200 for 1 h at room temperature. The slides sections were then rinsed three times in PBS for 10 min each and incubated in fluorophore-conjugated secondary antibodies: chicken polyclonal anti-goat, Alexa Fluor 488 (Invitrogen, Cat# A-21467, RRID: AB_141893) for meprin β; either chicken monoclonal anti-mouse, Alexa Fluor® 488 (Invitrogen Cat# A-21200, RRID: AB_2535786) or chicken polyclonal anti-rabbit, Alexa Fluor® 488 (Invitrogen, Cat# A21441, RRID: AB_2535859) for Villin; donkey monoclonal anti-rabbit, Alexa Fluor® 647 (Abcam Cat# ab150075, RRID: AB_2752244) for OPN, Caspase-3 and NFκB; donkey polyclonal anti-mouse, Alexa Fluor® 647 (Abcam Cat# ab150107, RRID: AB_2890037) for Bcl-2. For reasons of antibody compatibility, the Bcl-2 immunostaining counterstained with rabbit polyclonal anti-villin (Abcam Cat# ab52102, RRID: AB_883445) in WT kidney sections diluted 1:50. 4,6-Diamidino-2-phenylindole (DAPI) (Vector Laboratories Cat# SK-4100, RRID: AB_2336382) was used for nuclei staining. Prolong anti-fade reagent (Life Technologies, Carlsbad CA) was applied before mounting the sections with coverslips. The tissue sections were evaluated for meprins, OPN, Caspase-3, Bcl-2, and NFκB expression and localization using BZ-X700 Series. Fiji; Fiji ( Fiji Is Just ImageJ ) Fiji is an open source image processing package based on ImageJ. Fiji's main purpose is to provide a distribution of ImageJ with many bundled plugins. Fiji; Fiji ( Fiji Is Just ImageJ ) Fiji is an open source image processing package based on ImageJ. Fiji's main purpose is to provide a distribution of ImageJ with many bundled plugins.

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The future of banking is here, with the new Westpac Fiji Mobile Banking App. It’s fast, easy to use and secure.Take your banking with you, wherever you go. Use your smartphone or tablet to check your balance, transfer funds between your accounts or to a friend or family member. You can also pay your bills, top up your phone, or make an M-PAiSA payment (Vodafone Users only) – all via the easy to use, fast and secure Westpac Fiji Mobile Banking app.What you need to get started:- A personal transactional account with Westpac Fiji- Fiji mobile number that is linked to your accounts- Last four digits of your Handycard or Visa Debit Card- Be registered for Mobile Banking- A smart phone or tablet with iOS of 10.1 or more- Mobile data or Wi-FiWith Westpac Fiji Mobile Banking App you can:- Check your available balance.- View the last 25 transactions conducted in a month via mini statement.- Pay Bills to Westpac registered billers.- Pay other accounts in Westpac or other banks in Fiji.- Transfer money between your own accounts.- Top up your Mobile number or others within the same network as yours.- Make an M-PAiSA payment for Vodafone users only.- Download account statements in pdf format.Secure Banking:- The App is compatible with iOS greater than version 10.1- Use/create PIN length of 6 digit.- The App is enabled for Two Factor Authentication, a security feature whereby a six digit PIN will be sent to your mobile number currently registered for Mobile Banking via SMS and you will be required to enter this 6 digit PIN to authenticate and complete your transaction.It is highly advisable to always log out of the app when not in use. Do not store your App login PIN on your device. Keep your log in access a secret.Please read the Electronic Banking terms and conditions of use for more information on the product.If you are already signed up to Westpac mobile banking:- Download this App and follow the prompts for a one off App registration process. (after this you will be able to use the app using only